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NIH3T3 Cell Line

NIH3T3 is a cell line that was established in 1962 from the embryonic tissue of a Swiss mouse. NIH3T3 cells have a fibroblastic morphology and adherent cultural properties. NIH3T3 is susceptible to murine leukemia virus and a reliable transfection host. A reagent to transfect NIH3T3 cells is commercially available from Altogen Biosystems . NIH3T3 is one of the most often used fibroblastic lines in cell and molecular research. NIH3T3 cells can be used to study mouse viruses and human embryonic stem cells, as well as cultivate keratinocytes.
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Altogen Xenograft Models for Preclinical Drug Studies

Altogen Biosystems is a global producer and laboratory for the pharmaceutical industry. Biological products and additional goods include ELISA testing, optimization of preclinical research, 28-day generation of stably-transfected cells, IND applications and more. Dedicated to helping researchers with cutting-edge and efficient services, Altogen Biosystems strives to further modern laboratory techniques. Visit the Altogen website and catalog for xenograft models and other tools to assist in preclinical drug evaluations.
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Embryonic Cell Differentiation Studies with NIH3T3 Cells

NIH3T3 cells are crucial to a variety of embryonic stem cell research experiments, yet the cells themselves are murine fibroblasts, and are not the actual targets of such experiments. Rather, NIH3T3 cells serve as feeder layers in cell culture – providing necessary nutrients to the primary cells involved in research and enabling a realistic environment for the growth of stem cells in culture. Different kinds of proteins can be expressed if the cells are transfected with effective reagents , ensuring that custom research can proceed; should growth factors be necessary to the growth of different types of cells, simply transfecting the necessary sequences into cells will result in the proper expression. As such, other kinds of media can be structured with the NIH3T3 cell line, rendering it an important tool for biomedical research.
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NIH3T3 Transfection Reagent (Swiss Mouse Fibroblasts)

I n 1962 two scientists established the NIH3T3 cell line from embryonic Swiss mouse tissue. The derived cells are a type of murine embryonic fibroblast cell (MEF). The MEF cells  are used for the titration of viruses from mouse tissue and organs. They are also used to cultivate keratinocytes and to act as "feeder" cells in human embryonic stem cells. For research purposes, this cell line is a good transfection host because it is susceptible to leukemia and sarcoma viruses. Altogen Biosystems offers a  NIH3T3  cell line reagent that has a  high transfection efficiency - it is a lipid-based reagent that was detected by qRT-PCR to confirm high efficiency.  Transfection Reagent for NIH3T3 Cells (Swiss Mouse Fibroblast Cells, CRL-1658)
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