NIH3T3 is a cell line that was established in 1962 from the embryonic tissue of a Swiss mouse. NIH3T3 cells have a fibroblastic morphology and adherent cultural properties. NIH3T3 is susceptible to murine leukemia virus and a reliable transfection host. A reagent to transfect NIH3T3 cells is commercially available from Altogen Biosystems . NIH3T3 is one of the most often used fibroblastic lines in cell and molecular research. NIH3T3 cells can be used to study mouse viruses and human embryonic stem cells, as well as cultivate keratinocytes.