NIH3T3 cells are crucial to a variety of embryonic stem cell
research experiments, yet the cells themselves are murine fibroblasts, and are
not the actual targets of such experiments. Rather, NIH3T3 cells serve as
feeder layers in cell culture – providing necessary nutrients to the primary
cells involved in research and enabling a realistic environment for the growth
of stem cells in culture. Different kinds of proteins can be expressed if the
cells are transfected with effective reagents,
ensuring that custom research can proceed; should growth factors be necessary
to the growth of different types of cells, simply transfecting the necessary
sequences into cells will result in the proper expression. As such, other kinds
of media can be structured with the NIH3T3 cell line, rendering it an important
tool for biomedical research.
NIH3T3 is a cell line that was established in 1962 from the embryonic tissue of a Swiss mouse. NIH3T3 cells have a fibroblastic morphology and adherent cultural properties. NIH3T3 is susceptible to murine leukemia virus and a reliable transfection host. A reagent to transfect NIH3T3 cells is commercially available from Altogen Biosystems . NIH3T3 is one of the most often used fibroblastic lines in cell and molecular research. NIH3T3 cells can be used to study mouse viruses and human embryonic stem cells, as well as cultivate keratinocytes.
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